Foromo ea sekoahelo sa silicone ea poleiti e tebileng ea liliba tse 96

Silicone c ea poleiti e tebileng ea liliba tse 96Holim'a Hlobo

 

Ansix Baqapi ba Theknoloji ba Nepahetseng ka Litšenyehelo: Ho Tseba Molumo oa Silicone oa Sekoahelo sa Plate ea 96-Well Deep-Well

Lefatšeng la tlhahiso ea mahlale a bophelo le nang le mathata a maholo, moo ho tšepahala ho sa buisanoeng 'me likhatello tsa litšenyehelo li le khōlō, ho hloleha ha karolo e le 'ngoe ho ka sitisa ba limilione lipatlisisong. Mokhoa o mocha oa Ansix Tech oa projeke ea hlobo ea sekoahelo sa silicone o paka hore ho nepahala le ho ba le theko e tlase ha se lipheo tse ikhethileng.

 

Selelekela: Tiiso ea Bohlokoa Lilaboratoring tsa Kajeno

Sebakeng se maphathaphathe sa laboratori ea bongaka ea sejoale-joale, poleiti e bonolo ea seliba se tebileng sa 96 ke mosebetsi oa bohlokoa. Lipoleiti tsena, tsa bohlokoa bakeng sa tlhahlobo e phahameng ea tlhahiso, genomics, le ho sibolloa ha lithethefatsi, li itšetlehile ka motsoalle oa bohlokoa: sekoahelo sa silicone sa ho koala. Tsena ha se likoahelo tse bonolo empa ke likarolo tse entsoeng ka nepo tse lokelang ho theha tiiso e felletseng khahlanong le tšilafalo, ho mamella mocheso o feteletseng ho tloha polokelong ea -80°C ho isa ho 121°C autoclaving, le ho mamella matla a fetang 4000 g nakong ea centrifugation.

 

Phephetso ea ho etsa likoahelo tsena e lutse ho dual-half ea tsona e rarahaneng—li tlameha ho kobeha ka ho lekaneng ho etsa tiiso e phethahetseng empa e tšoarelle ka ho lekaneng bakeng sa tšebeliso e pheta-phetoang, ha tsohle li ntse li hlahisoa ka theko e etsang hore lits'ebetso tsa tšebeliso e le 'ngoe li khonehe bakeng sa lipatlisiso tsa lefats'e. Mona ke moo boiphihlelo ba Ansix Tech bo fetolang papali e rarahaneng ea tlhahiso hore e be tharollo e tšepahalang, e tsamaisoang ke boleng. Ka kopanyo e akaretsang ea saense e tsoetseng pele ea thepa, boenjiniere ba ho bolela esale pele, le lits'ebetso tse ntlafalitsoeng tsa tlhahiso, Ansix Tech e hlalositse bocha moruo le ts'epo ea ho hlahisa likarolo tsena tsa bohlokoa tsa laboratori.

 

Mokhahlelo oa 1: Moralo oa Motheo le Prototype

Leeto la sekoahelo sa silicone se sebetsang hantle le qala nako e telele pele thepa e qhibilihisitsoeng e ama tšepe. Bakeng sa sekoahelo sa liliba tse 96, mohato oa moralo o laoloa ke ho se sekisetse. maemo a tloaelehileng ea indasteri ea mahlale a bophelo, moo ho nepahala ha litekanyo ho lekanngoang ka li-micron 'me bohloeki ba thepa bo bohlokoa ka ho fetisisa.

 

Moralo oa Tlhahiso (DFM) e le Filosofi: Baenjiniere ba Ansix Tech ba qala projeke e 'ngoe le e 'ngoe ka tlhahlobo e felletseng ea DFM, ba tšoara moralo le tlhahiso e le phephetso e le 'ngoe, e kopaneng. Melao-motheo ea bohlokoa e sebelisoang e kenyelletsa ho boloka botenya ba lebota bo lekanang (hangata bo shebaneng le 1-3mm) ho pholletsa le gridi e rarahaneng ea li-grommet tse 96 tsa ho koala ho netefatsa hore lia pholile hantle le ho thibela matšoao a ho sotha kapa a ho teba. Lebota le leng le le leng le otlolohileng le entsoe ka sekhutlo se loketseng sa ho hula (1-2° ka lehlakoreng) ho nolofalletsa ho ntšoa ha hlobo ntle le ho senya silicone e bonolo. Ho feta moo, sebaka sa heke (moo thepa e kenang ka har'a mokoti oa hlobo) le mola oa karohano li behiloe ka mokhoa o bohlale libakeng tse seng tsa bohlokoa ho felisa liphoso tse bonahalang le ho fokotsa ts'ebetso ea kamora ho sebetsa.

 

Ho etsa mohlala bakeng sa netefatso: Pele Ansix Tech e itlama ho etsa disebediswa tsa tlhahiso tse theko e hodimo, e sebedisa mokhoa o potlakileng wa ho etsa mohlala ho tlosa kotsi morerong ona. Ho sebediswa mekgwa e kang dibopeho tsa aluminium tse entsweng ka mochini wa CNC, tse fanang ka ho nepahala ha ±0.05mm mme di ka tshehetsa dipotoloho tse fetang 500 tsa teko. Mokgahlelo ona ha se feela mabapi le netefatso ya sebopeho; ke sebaka sa teko se sebetsang. Dibopeho di feta netefatsong e matla bakeng sa katleho ya ho tiisa, ho hanyetsa dikhemikhale le ho mamella autoclave. Lintlha tsa indasteri di bontsha hore hoo e ka bang 35% ya diphoso tse ka bang teng tsa moralo di hlwauwa le ho lokiswa nakong ya mohato ona wa ho etsa mohlala, ho thibela ditjeo tse phahameng haholo nakong ya tlhahiso ya bongata.

 

Tafole ea 1: Tataiso ea Bohlokoa ea DFM bakeng sa Hlobo ea Sekoahelo sa Silicone

 

Mokhahlelo oa 2: Saense ea Lintho tse Bohlokwa le Boenjiniere ba ho Lepa Lintho Pele

Ho khetha thepa e nepahetseng ke qeto ea leano e lekanyang ts'ebetso, ho lumellana ha lintho tse phelang le litšenyehelo.

 

Elastomer e Khethiloeng: Rabara ea Silicone e Metsi (LSR): Bakeng sa ts'ebeliso ena, Ansix Tech e totobatsa Rabara ea Silicone e Metsi e hloekisitsoeng ka platinum e hloekileng haholo. Ho fapana le silicone e tiileng e tloaelehileng, LSR e fanoa e le sistimi ea likarolo tse peli e tsoakiloeng le ho kenngoa. E fana ka litšobotsi tse phahameng tsa phallo bakeng sa ho tlatsa hlobo e rarahaneng, botsitso bo ikhethang ba mocheso (ho mamella mocheso ho tloha ho -50°C ho ea ho feta 200°C), le durometer e tsitsitseng (bothata) ea bohlokoa bakeng sa matla a tšepahalang a ho tiisa. Ho feta moo, LSR e hloekisitsoeng ka platinum e fihlela litlhoko tse thata tsa FDA le USP Class VI bakeng sa ho lumellana ha lintho tse phelang, ho netefatsa hore ha ho na lintho tse ka lutlang tse sitisang lisampole tse bonolo tsa baeloji.

 

Tlhahlobo ea Phallo ea Mongobo (DFM Simulation): Ka ho sebelisa software e tsoetseng pele ea ketsiso joalo ka Autodesk Moldflow, baenjiniere ba theha karolo ea dijithale ea ts'ebetso ea ente. Tlhahlobo ena e bolela esale pele hore na LSR e tla phalla joang ka har'a li-runners le likoti tsa hlobo, e khetholla mathata a ka bang teng joalo ka maraba a moea (a ka bakang ho tlatsa ho sa fellang kapa li-bubble), mela ea weld (moo phallo ea thepa e arohaneng e kopanang teng, e bakang bofokoli bo ka bang teng), le pholiso e sa lekanang. Ka ho ntlafatsa sistimi ea heke le libaka tsa ho ntša moea ka mokhoa o hlakileng, Ansix Tech e fokotsa haholo palo ea lipotoloho tsa liteko le liphoso tsa 'mele, e fokotsa nako ea nts'etsopele le litšenyehelo tsa phetoho ea lisebelisoa. Lithuto li bontša hore prototyping e joalo ea dijithale e ka fokotsa liteko tsa 'mele ka ho feta 30%.

 

Mokhahlelo oa 3: Moralo le Tlhahiso ea Hlobo e Nepahetseng

Sebopeho ka bosona ke mosebetsi o motle oa boenjiniere bo nepahetseng, moo ntlha e 'ngoe le e 'ngoe e tlatsetsang boleng ba karolo ea ho qetela le bokhoni ba moruo ba projeke.

 

Khetho ea Tšepe ea Hlobo: Bakeng sa karolo e nang le bophahamo bo phahameng joalo ka sekoahelo sa liliba tse 96, ho tšoarella ke habohlokoa. Ansix Tech e sebelisa litšepe tse sa hloekang tse thatafalitsoeng kapa tse thatafalitsoeng ka botlalo, tse kang SS420 kapa S136, bakeng sa karolo e ka hare le e ka hare. Litšepe tsena li fana ka tekano e ntle ea ho sebetsa, ho ba bonolo ho polecha (ho fihlela qetellong ea seipone e netefatsang ho lokolloa ha karolo habonolo), le ho hanyetsa mafome khahlanong le lisebelisoa tsa LSR. Hangata ho thatafala ho bolokoa ho tloha ho HRC 48-52, ho fana ka nako e telele e hlokahalang bakeng sa bophelo ba hlobo bo ka fetang lipotoloho tse 500,000.

 

Boenjiniere ba Litsamaiso tsa Motheo:

 

Sistimi ea ho Pholisa: Ho pholisa ho nka 50-70% ea nako eohle ea potoloho ea ho enta ka ente. Ansix Tech e rala likanale tsa ho pholisa tse lumellanang tse latelang li-contour tsa sehlopha sa 96-cavity haufi-ufi kamoo ho ka khonehang. Ho boloka phallo ea metsi e sa tsitsang ka har'a likanale tsena (ka nomoro ea Reynolds e kaholimo ho 4000) ho bohlokoa bakeng sa ho eketsa katleho ea phetisetso ea mocheso. Sena se fokotsa nako ea potoloho ka kotloloho, e leng sesosa se seholo ka ho fetisisa sa litšenyehelo ka karolo.

 

Sistimi ea Runner & Heke: Sistimi ea runner e chesang hangata e sebelisoa bakeng sa LSR. Sistimi ena e boloka thepa e le boemong bo qhibilihisitsoeng ka har'a manifold, e felisa li-runner tse batang le litšila tse amanang le eona (tse ka bang tsa bohlokoa ka LSR e turang ea boemo ba bongaka). Liheke tsa nale-valve li fana ka taolo e nepahetseng holim'a ho tlatsa, ho netefatsa hore sebaka se seng le se seng se pakiloe ka ho lekana.

 

Sistimi ea ho ntša metsi: Ka lebaka la tlhaho e bonolo le e tenyetsehang ea LSR e phekotsoeng, sistimi ea ho ntša metsi e lokela ho ba bonolo haholo empa e tšepahala. Sistimi ea ho ntša metsi e nang le li-pin tse ngata holim'a sebaka se seholo, ka linako tse ling e kopantsoe le ho phatloha ha moea, e etsa bonnete ba hore sekoahelo se tenyetsehang sea tsoa ka mokhoa o hloekileng ntle le ho sotha.

 

Tafole ea 2: Lisebelisoa tsa Optimization bakeng sa Bokhoni ba ho Enta ka Ente

Mokhahlelo oa 4: Ho Tseba Mokhoa oa ho Bopa le Tiisetso ea Boleng

Ha hlobo e kentsoe ka har'a mochine o hatellang oa LSR o otlolohileng hantle, tlhokomelo e fetohela ntlafatsong ea ts'ebetso le taolong e matla ea boleng.

 

Mathata a Ts'ebetso le Ntlafatso: Ts'ebetso ea LSR e hlahisa litšitiso tse ikhethang. Ho teteana ha eona ho tlase ho hloka taolo e phethahetseng ho thibela ho fifala (thepa e feteletseng e kenellang likheong tsa hlobo). Pheko ea eona e itšetlehile ka mocheso, e leng se etsang hore taolo e nepahetseng ea mocheso oa hlobo (hangata e pakeng tsa 150-200°C) e be ea bohlokoa. Litsebi tsa Ansix Tech li ngola ka hloko paramethara e 'ngoe le e 'ngoe—lebelo la ente, khatello ea ho paka, nako ea pheko le mocheso. Sepheo ke ho fumana "sebaka se monate" moo nako ea potoloho e fokotsoang ntle le ho beha botšepehi ba karolo kotsing. Mohlala, ho fokotsa nako ea pheko ka 5% ka liprofaele tse ntlafalitsoeng tsa mocheso ho ka hlahisa lipoloko tse limilione selemong sa tlhahiso.

 

Setso sa Taolo ea Boleng: Boleng bo entsoe hore bo kene tšebetsong. Taolo ea Ts'ebetso ea Lipalopalo (SPC) e lekola litekanyo tsa bohlokoa ka nako ea sebele. Sehlopha se seng le se seng sa likarolo se hlahlojoa ka bongata: liteko tsa litekanyo ka mechini ea ho lekanya e kopanetsoeng (CMM), tlhahlobo ea durometer bakeng sa ho thatafala, tlhahlobo ea tiiso e sebetsang tlas'a khatello, le netefatso ea ho hanyetsa autoclave le lik'hemik'hale. Likarolo li etsoa le ho pakoa tikolohong e laoloang ea kamore e hloekileng (ISO 7 kapa ho feta) ho thibela tšilafalo, 'me karolo e' ngoe le e 'ngoe e ka lateloa morao ho sehlopha sa eona sa thepa e tala.

 

Mokhahlelo oa 5: Tšepiso ea Phano e Potlakileng

Tikolohong ea kajeno ea lipatlisiso tse potlakileng, ho potlakela 'marakeng ke monyetla oa tlholisano. "Ts'ebetso ea ho Fana ka Kapele" ea Ansix Tech ke mokhoa o hlophisitsoeng o kopanyang mekhahlelo ntle le ho tela boikitlaetso. Boenjiniere bo sebetsang ka nako e le 'ngoe, moo moralo oa hlobo o qalang ha liteko tsa ho qetela tsa thepa li ntse li tsoela pele, bo fokotsa libeke tse ngata ho tloha nakong ea nako. Li-simulation tse peli tsa dijithale li thibela liphetoho tsa moralo oa morao. Mekhoa e thehiloeng ea tlhahiso ea hlobo, mangolo a tšoanelehang le keketseho ea tlhahiso e netefatsa hore ho tloha ho hoamisoa ha moralo ho ea ho lihlooho tsa pele, ts'ebetso e ka boleloa esale pele ebile e potlakile.

 

Qetello: Boleng ba Boenjiniere ho Tiiso e 'Ngoe le e 'Ngoe

Tlhahiso ea sekoahelo sa silicone sa poleiti e tebileng ea liliba tse 96 ke mofuta o monyenyane oa tlhahiso ea sejoale-joale e tsoetseng pele. E hloka tšebelisano-'moho ea saense ea thepa, boenjiniere ba mechini le taeo ea ts'ebetso. Botsebi ba Ansix Tech bo lutse ho tsamaiseng bothata bona ka pono e hlakileng, e shebaneng le boleng. Ka ho etsa liqeto tse bohlale khethong ea thepa, ho sebelisa ketsiso ho thibela liphoso tse turang, ho rala liforomo bakeng sa katleho e kholo, le ho ntlafatsa motsotsoana o mong le o mong oa potoloho ea tlhahiso, ba fihlela sephetho se matla: ho fokotsa litšenyehelo tsohle tsa beng ba bareki ba bona ntle le micron ea ho sekisetsa boleng kapa ts'epo.

 

Bakeng sa lilaboratori le lik'hamphani tsa tlhahlobo lefatšeng ka bophara, sena se fetolela phepelo e tšepahalang ea sesebelisoa sa bohlokoa se ka sebelisoang ka litšenyehelo tse tsitsitseng, se nolofalletsang tsoelo-pele ea saense e ncha le e ntle moruong. Lefatšeng le bonolo la mahlale a bophelo, Ansix Tech ha e fane feela ka karolo, empa le motheo oa tšepo le boleng, o tiisitsoeng ka ho nepahala.

 

Ansix Tech Co Ltd.

Haeba o na le merero efe kapa efe e amanang le hlobo ea sekoahelo sa silicone ea poleiti e tebileng ea 96-well, o ka ikopanya le rona neng kapa neng. Re tla fetola mehopolo ea hau hore e be 'nete, re u lumelle ho phethahatsa litoro tsa hau, le ho fumana liodara tse kholo 'marakeng. Tlhahisoleseling ea rona ea ho ikopanya ke info@ansixtech.com. Kapa ikopanye le CTO ea rona, romella lengolo-tsoibila ho: stephen@ansixtech.com

 

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